dopamine da  (Elabscience Biotechnology)

Journal: Scientific Reports

Article Title: AAV-mediated intracerebral expression of an α-synuclein-targeting intrabody improves motor functions in aged rats

doi: 10.1038/s41598-025-34908-1

Figure Lengend Snippet: NAC32 intrabody enhances synaptic marker expression and dopamine levels in the striatum of aged rats. Aged rats (18 months) received bilateral injections of AAV1-NAC32 (expressing the NAC32 intrabody; n = 6) or AAV1-mCherry (control; n = 4) into the substantia nigra. (A) Fourteen weeks post-injection, striatal tissues were harvested for Western blot analysis of synaptic markers PSD95, synaptophysin, and TH, with β-actin as a loading control. The grouped blots shown were cropped from different exposures of distinct parts of the same gel. (B , C , D) Protein levels were quantified by densitometry, normalized to β-actin, and expressed as mean ± SEM. Significant differences were determined using the Student’s t-test (* p < 0.05, ** p < 0.01). Molecular weights (kDa) of the protein markers are indicated. (E) In parallel, dopamine levels in the striatum were measured using ELISA and normalized to total protein content, determined by the BCA assay. Data are presented as mean ± SEM; * p < 0.05 indicates statistical significance (Student’s t-test).

Article Snippet: The striatum homogenates were prepared as described in the section on Western blotting analysis and were subjected to quantification of total proteins using the BCA Protein Assay Kit (#23225, Pierce), measurement of dopamine with the DA ELISA Kit (#E-EL-0046, Elabscience), and assessment of 10 inflammation-related cytokines using the Quantibody ® Rat Inflammation Array 1 (#QAR-INF-1, RayBiotech), following the manufacturer’s instructions.

Techniques: Marker, Expressing, Control, Injection, Western Blot, Enzyme-linked Immunosorbent Assay, BIA-KA

Journal: Scientific Reports

Article Title: AAV-mediated intracerebral expression of an α-synuclein-targeting intrabody improves motor functions in aged rats

doi: 10.1038/s41598-025-34908-1

Figure Lengend Snippet: Cytokine profiles in the striatum of aged rats after AAV1-NAC32 treatment. Aged rats (18 months) were bilaterally injected with the virus AAV1-NAC32, which encodes the NAC32 intrabody ( n = 6), or with AAV1-mCherry, which encodes the mCherry fluorescent protein ( n = 4), in the substantia nigra. Fourteen weeks after the virus injection, striatum tissues were collected to quantify inflammation-related cytokines using a multiplexed sandwich ELISA-based quantitative array platform. The levels of seven pro-inflammatory cytokines (IFNγ, IL-1α, IL-1β, IL-2, IL-6, MCP-1, TNFα) and three anti-inflammatory cytokines (IL-4, IL-10, IL-13) were measured and normalized to the total protein level determined by the BCA assay. Data were plotted as mean values ± SEM. No significant differences for each measured cytokine were found between groups ( p > 0.05; Student’s t-test).

Article Snippet: The striatum homogenates were prepared as described in the section on Western blotting analysis and were subjected to quantification of total proteins using the BCA Protein Assay Kit (#23225, Pierce), measurement of dopamine with the DA ELISA Kit (#E-EL-0046, Elabscience), and assessment of 10 inflammation-related cytokines using the Quantibody ® Rat Inflammation Array 1 (#QAR-INF-1, RayBiotech), following the manufacturer’s instructions.

Techniques: Injection, Virus, Sandwich ELISA, BIA-KA